Electrostatically driven resonance energy transfer in “cationic” biocompatible indium phosphide quantum dots† †Electronic supplementary information (ESI) available: Detailed experimental methods, the synthesis and characterization of QDs, bioimaging, stability studies, control experiments, and the calculation of various parameters involved in the resonance energy transfer process etc. See DOI: 10.1039/c7sc00592j Click here for additional data file.
نویسندگان
چکیده
Indium Phosphide QuantumDots (InP QDs) have emerged as an alternative to toxic metal ion based QDs in nanobiotechnology. The ability to generate cationic surface charge, without compromising stability and biocompatibility, is essential in realizing the full potential of InP QDs in biological applications. We have addressed this challenge by developing a place exchange protocol for the preparation of cationic InP/ ZnS QDs. The quaternary ammonium group provides the much required permanent positive charge and stability to InP/ZnS QDs in biofluids. The two important properties of QDs, namely bioimaging and light induced resonance energy transfer, are successfully demonstrated in cationic InP/ZnS QDs. The low cytotoxicity and stable photoluminescence of cationic InP/ZnS QDs inside cells make them ideal candidates as optical probes for cellular imaging. An efficient resonance energy transfer (E 60%) is observed, under physiological conditions, between the cationic InP/ZnS QD donor and anionic dye acceptor. A large bimolecular quenching constant along with a linear Stern–Volmer plot confirms the formation of a strong ground state complex between the cationic InP/ZnS QDs and the anionic dye. Control experiments prove the role of electrostatic attraction in driving the light induced interactions, which can rightfully form the basis for future nano-bio studies between cationic InP/ZnS QDs and anionic biomolecules.
منابع مشابه
Electrostatically driven resonance energy transfer in "cationic" biocompatible indium phosphide quantum dots.
Indium Phosphide Quantum Dots (InP QDs) have emerged as an alternative to toxic metal ion based QDs in nanobiotechnology. The ability to generate cationic surface charge, without compromising stability and biocompatibility, is essential in realizing the full potential of InP QDs in biological applications. We have addressed this challenge by developing a place exchange protocol for the preparat...
متن کاملIdentifying short surface ligands on metal phosphide quantum dots† †Electronic supplementary information (ESI) available: Further experimental details and characterization data. See DOI: 10.1039/c6cp03564g Click here for additional data file.
The control and understanding of the chemical and physical properties of quantum dots (QDs) demands detailed surface characterization. However, probing the immediate interface between the inorganic core and the ligands is still a major challenge. Here we show that using cross-polarization magic angle spinning (MAS) NMR, unprecedented information can be obtained on the surface ligands of Cd3P2 a...
متن کاملEngineering a FRET strategy to achieve a ratiometric two-photon fluorescence response with a large emission shift and its application to fluorescence imaging† †Electronic supplementary information (ESI) available: Synthetic procedures and characterization data; additional spectroscopic data. See DOI: 10.1039/c4sc03883e Click here for additional data file.
متن کامل
AIEgens for dark through-bond energy transfer: design, synthesis, theoretical study and application in ratiometric Hg2+ sensing† †Electronic supplementary information (ESI) available: Synthetic procedures and characterizations, other experimental details. See DOI: 10.1039/c6sc04206f Click here for additional data file. Click here for additional data file. Click here for additional data file.
متن کامل